The master’s thesis in the Department of Clinical Chemistry at the College of Medicine at the University of Karbala discussed the nano method in preparing a new therapeutic compound for patients with rheumatoid arthritis. The thesis submitted by the researcher (Hanin Madloul Amanah) included the title “The nano method in preparing a new therapeutic compound for patients with rheumatoid arthritis”
The discussion committee consisted of:
Prof. Dr. Fardous Abbas Jaber, Chairman
Prof. Dr. Haider Subhi Sadiq, Member
Prof. Dr. Rana Majeed Hamid, Member
Prof. Dr. Fadhel Jawad Kazem Al-Ta’mah, Supervisor
Prof. Dr. Athir Hamid Awda, Supervisor
The study aimed to identify inflammatory and anti-inflammatory biochemical indicators for early detection of rheumatoid arthritis and diagnosis to prevent the development of type 2 diabetes (T2DM), and the association between signs of disease-related variables. Artemisinin, a drug that is effectively delivered using hyaluronic acid to encapsulate nanonucleosomes, was used to demonstrate the potential of this approach in the treatment of rheumatoid arthritis. Using molecular PCR, different factors, such as interleukin (IL-4), chemokine (-Ligand-2121 (activator. MMP2)), matrix metalloproteinase-2, and COX2 (cyclooxygenase-2), were evaluated in both peripheral blood mononuclear cells (PBMCs) before and after treatment. The study also highlights the potential of hyaluronic acid-encapsulated nanoparticles as an effective delivery system for artemisinin targeting rheumatoid arthritis and type 2 diabetes derived from peripheral blood mononuclear cells. The nanocomposite of hyalo-neo-artemisinin nanoparticles showed important properties, including stability and controlled drug release. The incorporation of hyaluronic acid enhanced the targeting and therapeutic effect of these niosomes. The synthesized hyalo-neo-artemisinin nanoparticles nanocomposite also showed significant reductions in the pro-inflammatory cytokine CCL-21, and inflammation-related genes (MMP2, 2TIMP, and COX2) in peripheral blood cells (peripheral blood Moncler cells) extracted from the blood of RA patients. This suggests a promising reduction in inflammation and enhancement of overall anti-inflammatory and antioxidant defenses.
