The College of Medicine, in cooperation with the Center for Continuing Education at the University of Karbala, organized a workshop on Elisa Technology on Sunday, 16/2/2020. The workshop was run by M. Nada Nizar Ahmed, MSc Clinical Chemistry.
((This workshop aims to increase the experiences of teachers and students of studies and encourage them to benefit from this technique in their research))
And I confirmed within her reach explaining the importance of this topic
((Being one of the widely used diagnostic laboratory techniques in most pathology analyzes laboratories, especially for the diagnosis of viruses for the following reasons:
- Analysis of a large number of samples.
- It takes relatively little time.
- 3.High Sensitivity of 99%.
It showed the principle of ELISA technology that this technology is based on the principle of Enzyme immunoassay EIA. In this technique, the antibody or antigens are attached to the drilling of a plastic plate called a Microtiter plate. Patient serum samples and control samples are placed in the microtiter plate pits and an association between antibodies and antigens is established. After an hour after incubation at room temperature, the plate was washed with a special washing solution to remove unrelated materials. An enzyme conjugate is then added to the antibody and incubated for half an hour. After another washing plan, the substrate is added and the plate is incubated for 20 minutes, as color (usually blue) appears in the pits. Then add a stop solution, where the color changes from blue to yellow. The color intensity is measured using a 450 nm wavelength spectrometer. The antibody concentration or antigen to be measured is directly proportional to the intensity of the color.
